The CHOP Microbiome Center supports planning microbiome projects, DNA purification, library preparation, high throughput sequence analysis, and bioinformatic analysis of the output.
The Analytical Core provides expertise in large-scale bioinformatics analysis of microbiome datasets. Core services start with a thorough quality check and review of sequencing results. This includes removal of host genomic sequences and other DNA added during the sequencing protocol. Automated pipelines assess the bacterial, fungal, and viral taxa found in each sample. Alignments to genes of known function are summarized, and the data are queried against a curated collection of full genomes for microbes of interest.
The High-throughput Screening (HTS) core provides the Perelman School of Medicine community with professional HTS screening services to identify genes or organic small molecule modulators of signaling pathways, cellular phenotypes, and protein function in models of human disease.
Many trillions of microbes—too small to be seen by the naked eye–live in our guts and elsewhere on our bodies. These microbes contribute to health by helping with digestion, guiding growth of our immune systems, and shouldering out invading pathogens. People differ greatly in the composition of their microbiota, influencing us each in unique ways.
The Sequencing Core provides expertise in next-generation sequencing of microbiome samples. Services include sample aliquoting, DNA extraction, quantitative PCR, library preparation, and sequencing. All protocols and workflows are optimized for individual sample types.
The Quantitative Proteomics Resource Core (QPRC) provides investigators access to the most advanced high resolution mass-spectrometry-based proteomics technologies. These approaches are implemented with a broad variety of mass-spectrometry-based experiments to characterize and quantify proteins from complex biological samples.
The goals of the Acute Care Biobanking Core are to encourage and facilitate microbiome- focused research in the pathogenesis, diagnosis and treatment of patients with critical illness. Many patients who are critically ill are subject to processes and complications with microbially-driven or infectious mechanisms.
The Human Intervention Core offers a wide array of services to assist with the design and implementation of microbiome studies. The core can assist with longitudinal studies as well as pilot studies. Pilot studies can be rapidly implemented with human intervention core staff, project managers and research coordinators, to conduct these studies.
Some 100 trillion beneficial microorganisms populate your body inside and out. Various species are healthy, and aid in processes like digestion, and strengthening the immune system. However, what would happen if humans did not have this symbiotic relationship with commensal bacteria? The University of Pennsylvania has launched The Penn Gnotobiotic Mouse Facility (PGMF) in order to aid new research in this area, and provide investigators with germ-free mice.
Dear Colleagues,
We are pleased to announce the 2020 Symposium on High-throughput Screening Technologies at Penn will take place in the Biomedical Research Building (BRB) Gaulton Auditorium on:
Thursday, April 30th from 9 am to 3:30 pm
Join us to learn about high-throughput screening projects and platforms available at Penn and how high-throughput screening can be used in basic and translational medicine.
Please join us on October 3rd for the 6th Annual PennCHOP Microbiome Program Symposium. This year, we are collaborating with the Abramson Cancer Center! Please see below for the agenda.
Thursday, October 3rd, 2019
8 am – 7 pm
BRB II/III, Gaulton Auditorium
View the flyer here
Symposium registrants may submit 40” x 60” posters. Poster abstracts (500 words max.) must be submitted to pcmp [at] pennmedicine.upenn.edu by 9/23/19
The Pittsburgh Rust Belt Microbiome Conference will take place November 4 and 5, 2019 at Carnegie Mellon University. For more information, head to https://events.mcs.cmu.edu/rbm2019/ or check out the flyer attached here.
