The Quantitative Proteomics Resource Core (QPRC) provides investigators access to the most advanced high resolution mass-spectrometry-based proteomics technologies. These approaches are implemented with a broad variety of mass-spectrometry-based experiments to characterize and quantify proteins from complex biological samples.
The High-throughput Screening (HTS) core provides the Perelman School of Medicine community with professional HTS screening services to identify genes or organic small molecule modulators of signaling pathways, cellular phenotypes, and protein function in models of human disease.
The goals of the Acute Care Biobanking Core are to encourage and facilitate microbiome- focused research in the pathogenesis, diagnosis and treatment of patients with critical illness. Many patients who are critically ill are subject to processes and complications with microbially-driven or infectious mechanisms.
The Human Intervention Core offers a wide array of services to assist with the design and implementation of microbiome studies. The core can assist with longitudinal studies as well as pilot studies. Pilot studies can be rapidly implemented with human intervention core staff, project managers and research coordinators, to conduct these studies.
Many trillions of microbes—too small to be seen by the naked eye–live in our guts and elsewhere on our bodies. These microbes contribute to health by helping with digestion, guiding growth of our immune systems, and shouldering out invading pathogens. People differ greatly in the composition of their microbiota, influencing us each in unique ways.
Some 100 trillion beneficial microorganisms populate your body inside and out. Various species are healthy, and aid in processes like digestion, and strengthening the immune system. However, what would happen if humans did not have this symbiotic relationship with commensal bacteria? The University of Pennsylvania has launched The Penn Gnotobiotic Mouse Facility (PGMF) in order to aid new research in this area, and provide investigators with germ-free mice.
The CHOP Microbiome Center supports planning microbiome projects, DNA purification, library preparation, high throughput sequence analysis, and bioinformatic analysis of the output.
The PennCHOP Microbiome Program, along with its matching partners in the School of Veterinary Medicine and the Center for AIDS Research, is proud to announce the next call for projects as a part of the Microbiome Pilot & Feasibility Program.
Application Deadline: 3/16/18
In the second lecture of our spring series, Kyle Bittinger PhD discussed the diversity of bacterial communities. Diversity can mean the number of species observed in each sample (alpha diversity) or the number of species shared between samples (beta diversity). The way diversity is used to analyze bacterial communities was discussed, as well as special topics such as UniFrac distance and principal coordinates analysis.
We would like to congratulate those selected for 2017 Pilot & Feasibility Project funding, and thank all those who applied.
Please click here to see a full project listing.
April 13, 2017
This introductory lecture, led by Kyle Bittinger PhD, covered the process of turning DNA sequence data into information about bacterial communities. Some approaches are purely numerical, while others involve associating the sequences with genus or family names. Methods of creating “operational taxonomic units” and the dangers of making taxonomic assignments were also be discussed.
“Transfer: Dissecting the interplay of MCH/HLA loci, the microbiota and autoimmune diabetes”
“Longitudinal analysis of respiratory tract microbiome change and sequence-based infection diagnosis during mechanical ventilation”
“Role of Bacterial Urease in Host and Gut Microbiota Amino Acid Metabolism”
